A. Wiedlocha

Translocation of fibroblast growth factor 1 (FGF-1) from the cell exterior to the cytosol is a new principle for transmembrane signalling and so far we do not know much about the mechanism of translocation. We are using our knowledge from the toxin work in attempts to elucidate the mechanism. It has proven difficult to gain general acceptance that such a signalling mechanism exists, and we have therefore so far concentrated our efforts on establishing that the growth factor is really translocated from the exterior of cells into their cytosol and nucleus. Once we know more about the mechanism of translocation such that the whole concept is more widely accepted, we intend to study the mechanism by which the internalised growth factor stimulates DNA synthesis. We also plan to test if other growth factors have an intracellular mode of action in addition to their ability to signal through a transmembrane receptor.

Antoni Wiedlocha's Group click to enlarge image

Contact information:
Email: Antoni.Wiedlocha@rr-research.no
Department of Biochemistry, Institute for Cancer Research
The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
Phone +47 22 78 19 30 (Wiedlocha), Switchboard: +47 22 93 40 00

Wiedlocha A, Nilsen T, Wesche J, Sørensen V, Ma?ecki J, Marcinkowska E, Olsnes S (2005)
Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1
Mol Biol Cell, 16 (2), 794-810
PubMed 15574884

Wesche J, Malecki J, Wiedlocha A, Skjerpen CS, Claus P, Olsnes S (2006)
FGF-1 and FGF-2 require the cytosolic chaperone Hsp90 for translocation into the cytosol and the cell nucleus
J Biol Chem, 281 (16), 11405-12
PubMed 16495214

Nilsen T, Rosendal KR, Sørensen V, Wesche J, Olsnes S, Wied?ocha A (2007)
A nuclear export sequence located on a beta-strand in fibroblast growth factor-1
J Biol Chem, 282 (36), 26245-56
PubMed 17616529

Zhen Y, Sørensen V, Jin Y, Suo Z, Wiedlocha A (2007)
Indirubin-3'-monoxime inhibits autophosphorylation of FGFR1 and stimulates ERK1/2 activity via p38 MAPK
Oncogene, 26 (44), 6372-85
PubMed 17533378